Should I keep my kitten in my room

She has toys all over the room she climbs up a ladder I made for her she has food and water but I want to keep her safe from the wild puppy until she's big enough to use her claws or teeth
What age should she be before I let her roam alone in the house???

Yes, you should keep her in your room. You also need to train the puppy not to be rough with the kitten. That may involve keeping your dog on a leash whenever the kitten is around. Your kitten should not be in a position where it has to defend itself.