No; they've been together for about a year and a half. They have monitored "together time" once in a while, but only in a one-on-one manner -- they're rarely all three together.
I have two male cats (neutered) at my mother's house. She already had a female cat. Right now, they have to be separated because the males will chase her and generally make her feel stressed (which is easy to do to her). I feel awful that we have to keep her locked up in a room alone for most...